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The separation principle in SEC is based within the fully, or partly penetrating with the large molecular excess weight substances on the sample to the porous stationary-period particles all through their transportation by means of column. The cell-section eluent is selected in such a way that it absolutely stops interactions While using the statio

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Glass: Much less well known and less force tolerant. Having said that, glass HPLC columns are employed when inert surfaces are The key characteristic that is necessary for Specific chemical or Organic purposes.Two pistons are set in collection in twin-piston in-collection pumps as per the following schematic diagram. Within this system, separate pi

SEC is effective also inside of a preparative way by trapping the smaller sized molecules inside the pores of a particles. The bigger molecules simply pass from the pores as They are really much too big to enter the pores. Larger sized molecules hence movement in the column a lot quicker than lesser molecules: that is certainly, the more compact th